Molecular Characterization of Mid-late Maturing Sugarcane Clones by Using Microsatellite Markers

A total of 56 alleles were obtained, which 41 shared and 15 unique allelic variants generated as the amplified product by polymerase chain reaction using eleven primer pairs. The PIC values revealing diversity frequency among genotypes varied from 0.36 in case NKS 57 to 0.90 1 with an average 0.62. similarity coefficients genetic respect size products targeted regions genome 0.518 0.857 for pairwise combinations amongst thirteen entries under evaluation. Two Clusters obtained when phenol line was drawn at twenty-five units. Cluster I consist ten genotypes. tri-genotypic cluster II consists three When level drew fifty unit clusters further divided into sub-clusters. It is concluded that SSR marker a very reliable approach identifying diverse genotype(s) where phenotypic cultivars leads difficulty while selecting parents hybridization.